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Molecular identification of two genotypes of mumps virus causing two regional outbreaks in Asturias, Spain

J A Boga1, M de Oña, A Fernández-Verdugo

  • 1Servicio de Microbiología, Hospital Universitario Central de Asturias, Oviedo, Spain. joseantonio.boga@sespa.princast.es

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|April 29, 2008
PubMed
Abstract

Insights

Mumps virus strains not matching vaccine types caused outbreaks in vaccinated individuals. Phylogenetic analysis identified two distinct mumps virus strains, highlighting the need for updated vaccination strategies against evolving mumps genotypes.

Area of Science:

  • Virology
  • Epidemiology
  • Public Health

Background:

  • Despite widespread mumps vaccination programs, sporadic cases and outbreaks continue to occur globally.
  • Understanding the genetic diversity of circulating mumps virus strains is crucial for effective disease control.

Purpose of the Study:

  • To perform phylogenetic characterization of mumps virus strains responsible for two regional outbreaks in Asturias, Spain.
  • To compare the genetic makeup of outbreak strains with those used in current vaccination protocols.

Main Methods:

  • Mumps virus strains were isolated from patient samples during the Asturias outbreaks.
  • Sequencing of the SH (Small Hydrohydrophobic) gene was performed for phylogenetic analysis.
  • Sequences were aligned with homologous sequences from representative strains of known mumps genotypes.

Main Results:

  • Two distinct mumps virus strains, designated Ast/SP02 and Ast/SP07, were identified.
  • Ast/SP02 belonged to genotype H, while Ast/SP07 was phylogenetically related to a reference strain of a newly emerging genotype.
  • Both identified strains belonged to genotypes distinct from the Jeryl-Lynn strain (genotype A) used in the vaccination program.

Conclusions:

  • Mumps virus strains that differ genetically from vaccine strains can precipitate outbreaks, even in highly vaccinated populations.
  • The findings underscore the potential for vaccine escape due to viral evolution and the emergence of new genotypes.

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