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Multiwalled carbon nanotube modified screen-printed electrodes for the detection of p-aminophenol: optimisation and
Pedro José Lamas-Ardisana1, Paula Queipo, Pablo Fanjul-Bolado
1Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
Abstract:
Carboxylated multiwalled carbon nanotubes (MWCNT-COOH) were used to modify the working electrode surface of different screen-printed electrodes. The effect of this modification on the electrodic characteristics (double layer capacitance, electroactive area and heterogeneous rate constants for the electron transfer) was evaluated and optimized for the cyclic voltammetric determination of p-aminophenol. The enzymatic hydrolysis of p-aminophenylphosphate was employed for the quantification of alkaline phosphatase, one of the most important label enzymes in immunoassays. Finally, ELISA assays were carried out to quantify pneumolysin using this enzymatic system. Results obtained indicated that low superficial densities of MWCNT-COOH (0.03-0.06 microg mm(-2)) yielded the same electrodic improvements but with better analytical properties.
