Related Experiment Video
Updated: Jul 5, 2026
An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
Development and validation of a UPLC method for quality control of rhubarb-based medicine: fast simultaneous
Jiabo Wang1, Huifang Li, Cheng Jin
1PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, PR China.
Abstract:
A reverse phase ultra performance liquid chromatography (UPLC) method was developed for the rapid quantification of five anthraquinone derivatives (aloe-emodin, rhein, emodin, chrysophanol and physcion) in rhubarb using a Waters Acquity BEH C18, 50 mm x 2.1 mm, 1.7 microm column. The runtime was as short as 3 min. The influence of flow rate and column temperature on resolution was investigated. The method was validated according to the regulatory guidelines with respect to precision, accuracy, linearity and robustness. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The proposed method was found to be reproducible and convenient for quantitative analysis of five anthraquinone derivatives in three species of rhubarb and related preparations.
More Related Videos
05:59An In Vitro Dissolution Determination of Multi-Index Components in Tibetan Medicine Rhodiola Granules
Published on: November 4, 2022
10:22Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Published on: June 30, 2020