The effects produced by acidified serum upon adipocytic conversion in 3T3-F442A cells

The acidified fetal bovine serum (FBS) produces a factor which inhibits the adipose differentiation of murine fibroblasts 3T3-F442A. In this work, we studied if the inhibitory factor (IF) has any effect on the proliferation of 3T3-F442A cells. Our data showed an increment in the number of cells cultured in the presence of IF. We do not know how the IF maintains the cell proliferation. Furthermore, we investigated if IF acts on the resting state (Go) of the 3T3-F442A. We found that the IF prevented the adipose differentiation in the state (Go), this data suggests that the 3T3-F442A in this state (Go) were not yet compromised to difference to adipocytes. We also showed that bovine serum (BS) had an inhibitory activity too, but this was lower than FBS. This report suggests that the IF may play a role during the development of the adipose tissue.