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[Parasite-origin IgE-dependent histamine-releasing factors in inducing histamine release from sensitized mast cells]

Xiao-Xiang Chen1, Xu-Chu Hu, Jing Xu

  • 1Department of Medical Microbiology, Guangzhou Medical College, Guangzhou 510182, China.

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases
|April 30, 2008
PubMed
Abstract

Insights

Recombinant histamine-releasing factors (HRFs) from parasitic worms were obtained and shown to induce histamine release from mast cells. This suggests a role for these parasite-derived HRFs in type I hypersensitivity reactions during parasitic infections.

Area of Science:

  • Parasitology
  • Immunology
  • Molecular Biology

Background:

  • Parasitic infections can trigger allergic responses.
  • Histamine release from mast cells is a key mediator of allergic reactions.
  • Understanding parasite-derived factors involved in these responses is crucial.

Purpose of the Study:

  • To produce recombinant IgE-dependent histamine-releasing factors (HRFs) from Schistosoma japonicum (rSjHRF) and Clonorchis sinensis (rCsHRF).
  • To investigate the capacity of these recombinant HRFs to induce histamine release from sensitized rat mast cells.

Main Methods:

  • Cloning and expression of SjHRF and CsHRF genes in E. coli.
  • Purification of soluble recombinant proteins rSjHRF and rCsHRF.
  • Incubation of mast cells with recombinant HRFs and measurement of released histamine using spectrofluorometry.

Main Results:

  • Successfully constructed and purified recombinant rSjHRF and rCsHRF.
  • Both rSjHRF and rCsHRF induced dose-dependent histamine release from sensitized mast cells.
  • Maximal histamine release was observed at 35 minutes, with significant percentages released at 150 mg/L concentration.

Conclusions:

  • Recombinant parasite-derived IgE-dependent HRFs effectively induce histamine release from mast cells.
  • These findings suggest a role for parasite HRFs in type I hypersensitivity in hosts with parasitic infections.

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