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Related Experiment Video

Updated: Jul 5, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

Published on: October 15, 2015

[Study on excess sludge solubilization in S-TE process under microaeration].

Yan-Wei Shi¹, Xiao-Ming Li, Wei-Na Zhao

¹College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. huawei2007@126.com

Huan Jing Ke Xue= Huanjing Kexue

|April 30, 2008

Summary

Thermophilic enzyme pretreatment (S-TE) enhances excess sludge solubilization by breaking down organic matter. This process, aided by bacteria and microaeration, increases soluble chemical oxygen demand and volatile fatty acids for better anaerobic digestion.

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Area of Science:

Environmental Biotechnology
Wastewater Treatment
Biochemical Engineering

Background:

Excess sludge solubilization is crucial for efficient wastewater treatment.
Thermophilic enzyme pretreatment (S-TE) offers a potential method for sludge breakdown.
Understanding the mechanisms and optimal conditions for S-TE is essential.

Purpose of the Study:

To investigate the effect of S-TE on excess sludge solubilization.
To analyze chemical component changes during S-TE under microaeration and varying temperatures.
To evaluate the role of thermophilic bacteria in the S-TE process.

Main Methods:

S-TE pretreatment involving enzyme-catalyzed and thermal-hydrolyzed reactions.
Addition of thermophilic bacteria (Bacillus stearothermophilus sp. AT06-1).
Operation under microaeration conditions at different temperatures.
Analysis of total suspended solid (TSS), volatile suspended solid (VSS), soluble chemical oxygen demand (SCOD), volatile fatty acid (VFA), and protease activity.

Main Results:

S-TE involves depolymerization by exoenzymes and disintegration of cell walls and organic matter.
Thermophilic bacteria enhanced the solubilization process compared to the control.
Optimal temperature (65°C) yielded high TSS (34.09%) and VSS (24.16%) solubilization rates within 2 days.
Microaeration produced maximal SCOD (4,531 mg/L) and VFA (2,319 mg/L), promoting protease activity and protein hydrolysis.

Conclusions:

S-TE is an effective pretreatment method for excess sludge solubilization.
The process is enhanced by thermophilic bacteria and microaeration.
The generated SCOD and VFA are beneficial for subsequent anaerobic digestion.
Optimal conditions significantly improve sludge solubilization efficiency.