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Related Concept Videos

The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

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Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

Making recombinant extracellular matrix proteins.

Florence Ruggiero1, Manuel Koch

  • 1Institut de Biologie et Chimie des Protéines, UMR CNRS 5086, Université de Lyon, Université Lyon 1, IFR 128 Biosciences Gerland, 7 passage du Vercors, Lyon Cedex 07, France. f.ruggiero@ibcp.fr

Methods (San Diego, Calif.)
|April 30, 2008
PubMed
Summary
This summary is machine-generated.

Producing recombinant extracellular matrix proteins, especially collagens, is vital for biomedical research. This review guides selecting optimal expression systems and protocols for efficient protein production.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Biomedical Engineering

Background:

  • Extracellular matrix (ECM) proteins are crucial for tissue structure and function.
  • Recombinant protein production is essential for studying ECM protein structure and function.
  • The biomedical industry shows significant interest in heterologous expression of ECM proteins, particularly collagens.

Purpose of the Study:

  • To review critical factors for reliable and cost-effective recombinant ECM protein production.
  • To evaluate advantages and drawbacks of various expression systems.
  • To describe protocols facilitating efficient recombinant ECM production, with a focus on collagens.

Main Methods:

  • Review of existing literature and methodologies for recombinant protein expression.
  • Analysis of key considerations: construct design, cloning strategies, expression vectors, transfection methods, and host cell systems.
  • Appraisal of different expression systems for ECM protein production.

Main Results:

  • Most large, modular, and multimeric ECM proteins can be successfully produced in various systems.
  • Specific structural features and complex post-translational modifications of collagens present challenges in their recombinant production.
  • Factors influencing successful production include careful selection of expression systems and optimization of protocols.

Conclusions:

  • Choosing appropriate strategies for construct design, expression systems, and protocols is key for efficient recombinant ECM protein production.
  • Recombinant collagen production requires addressing specific structural and post-translational modification challenges.
  • This review offers guidance for researchers and the biomedical industry in producing recombinant ECM proteins.