Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modular extracellular matrices: solutions for the puzzle.

Monica A Serban1, Glenn D Prestwich

  • 1Department of Medicinal Chemistry and Center of Therapeutic Biomaterials, The University of Utah, 419 Wakara Way, Salt Lake City, UT 84108-1257, USA.

Methods (San Diego, Calif.)
|April 30, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microneedle Designs for Trans-Tympanic Delivery of Topical Ototherapeutics.

ACS biomaterials science & engineering·2025
Same author

Stability Studies of a Tetraethyl Orthosilicate-Based Thixotropic Drug Delivery System.

Pharmaceutics·2024
Same author

The Application of Fluorescence Anisotropy for Viscosity Measurements of Small Volume Biological Analytes.

Journal of experimental and theoretical analyses·2024
Same author

<i>In vitro</i> characterization of novel hyaluronan-antioxidant conjugates as potential topical therapeutics against hearing loss.

Frontiers in pharmacology·2024
Same author

Hyaluronic acid-ibuprofen conjugation: a novel ototherapeutic approach protecting inner ear cells from inflammation-mediated damage.

Frontiers in pharmacology·2024
Same author

Colorimetric Properties of <i>Bombyx mori</i> Silk Fibroin.

ACS biomaterials science & engineering·2023
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Researchers developed semi-synthetic extracellular matrices (sECMs) from hyaluronan derivatives for advanced 3-D cell culture. These biomimetic materials support in vitro and in vivo tissue formation, addressing limitations in current cell culturing techniques.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Traditional 2-D cell culture is being superseded by 3-D methods.
  • Current 3-D culture lacks a versatile, biocompatible material for seamless in vitro-to-in vivo translation.
  • Replicating native extracellular matrix (ECM) complexity is crucial for advanced cell applications.

Purpose of the Study:

  • To develop modular, biomimetic materials for 3-D cell culture.
  • To create a versatile, reproducible, and affordable matrix for diverse biological applications.
  • To enable seamless transition from in vitro research to in vivo applications.

Main Methods:

  • Development of semi-synthetic ECMs (sECMs) using hyaluronan derivatives.
  • Formation of covalently crosslinked, biodegradable hydrogels for 3-D cell encapsulation.

Related Experiment Videos

  • Engineering sECMs with specific biological cues to mimic native ECM environments.

    • Main Results:

    • Successfully created modular sECMs suitable for 3-D culture of primary and stem cells.
    • Demonstrated the utility of sECMs for in vitro cell expansion and in vivo tissue formation.
    • Showcased sECM adaptability for applications including controlled stem cell differentiation, wound healing, and tissue repair.

    Conclusions:

    • The developed sECMs offer a versatile and reproducible platform for advanced 3-D cell culture.
    • These biomimetic materials facilitate tissue engineering and regenerative medicine applications.
    • sECMs provide a promising solution for drug discovery, toxicology, and therapeutic interventions.