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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: Jul 5, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Cancer proteomics by quantitative shotgun proteomics.

Emily I Chen¹, John R Yates

¹Department of Cell Biology, 10550 North Torrey Pines Road, SR11, The Scripps Research Institute, La Jolla, CA 92037, USA.

Molecular Oncology

|April 30, 2008

Summary

Investigating the molecular basis of cancer requires understanding complex cellular interactions. Shotgun proteomics offers a powerful approach to analyze protein expression and signaling pathways, advancing cancer research.

Keywords:

cancer cells mass spectrometry protein profiling quantitative proteomics shotgun proteomics

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

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Last Updated: Jul 5, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

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Published on: February 27, 2015

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

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Published on: August 2, 2018

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Area of Science:

Oncology
Proteomics
Molecular Biology

Background:

Cancer research faces challenges in determining the biological basis of cancer development due to its heterogeneity.
Understanding cancer's molecular underpinnings necessitates studying intricate protein interactions, cell signaling, and the tumor microenvironment.

Purpose of the Study:

To review the advancements in shotgun proteomics technologies for investigating the molecular basis of cancer development.
To highlight the potential of large-scale proteome analysis in understanding cancer transformation mechanisms.

Main Methods:

Review of recent progress in shotgun proteomics technologies.
Discussion of mass spectrometry and bioinformatics tools for protein interaction and signaling pathway analysis.

Main Results:

Shotgun proteomics enables qualitative and quantitative interrogation of dynamic protein-protein interactions.
Advances in technology facilitate the study of differential regulation of cellular signaling pathways in tumor development.

Conclusions:

Shotgun proteomics is a valuable tool for exploring the molecular mechanisms of cancer.
Technological progress in proteomics provides significant opportunities for cancer research and understanding tumor development.