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Updated: Jul 5, 2026

Generating Genetically Modified Plasmodium berghei Sporozoites
Published on: May 5, 2023
Processing of the circumsporozoite protein in infected hepatocytes is not dependent on aspartic proteases
S E Bongfen1, S Balam, R Torgler
1Department of Biochemistry, University of Lausanne, Ch. des Boveresses, Epalinges, Switzerland.
Abstract:
CD8(+) T cells play a major role in the protective immune response against the liver stage of malaria. It was previously shown that the circumsporozoite protein (CSP) is processed and presented to specific T cells by both traversed and infected hepatocytes, but their respective antigen processing requirements were not completely defined. In the present study, we show that in vitro processing of the Plasmodium berghei CSP by infected mouse primary hepatocytes is exclusively dependent on proteasomes, while aspartic proteases are also needed in the case of traversed hepatocytes.
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