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Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
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Compression and crevasses in vitreous sections under different cutting conditions

H-M Han1, B Zuber, J Dubochet

  • 1Laboratoire d'Analyse Ultrastructurale, Bâtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland. hongmei.han@yahoo.com.cn

Journal of Microscopy
|May 1, 2008
PubMed

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...