Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

cAMP-dependent Protein Kinase Pathways

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Erratum: [Corrigendum] Engineered AXL<sup>-ECD</sup>-Fc variants that abolish the AXL/Gas6 interaction suppress tumor cell migration.

Oncology letters·2026

Same author

Plasma metabolic signature of cardiovascular-kidney-metabolic syndrome and incident stroke: A nationwide prospective cohort study.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2026

Same author

Pan-filovirus activity of an IGF2-fused monoclonal antibody: Impairment by IGF1R cross-engagement and rescue via IGF2 Y27L mutation.

Virus research·2026

Same author

Neuropsychiatric disorders in children of mothers with polycystic ovary syndrome: a systematic review and meta-analysis.

BMC psychiatry·2026

Same author

Characterization and antitumor activities of polysaccharides derived from Inonotus sanghuang against cancers of the liver and lungs.

International journal of biological macromolecules·2026

Same author

Antibody Screening and Binding Prediction Analysis Targeting Stx2.

Antibodies (Basel, Switzerland)·2026

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.

Ning Zhu¹, Jian Cui, Chunxia Qiao

¹Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, China.

Cellular & Molecular Immunology

|May 1, 2008

Summary

Cyclic adenosine monophosphate (cAMP) inhibits macrophage development by suppressing M-CSF-induced MAPKs activation. This study explores cAMP

More Related Videos

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Published on: March 30, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Mesenchymal Stem Cell Regulation of Macrophage Phagocytosis; Quantitation and Imaging

Published on: July 16, 2021

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Published on: March 30, 2018

Area of Science:

Immunology
Cell Biology
Molecular Biology

Background:

Macrophage development is regulated by macrophage colony-stimulating factor (M-CSF), which induces mitogen-activated protein kinases (MAPKs) activation.
Cyclic adenosine monophosphate (cAMP) is known to inhibit MAPKs activation in response to inflammatory stimuli.

Purpose of the Study:

To investigate the effects of cAMP on M-CSF-induced MAPKs activation.
To determine the impact of cAMP on macrophage development.

Main Methods:

Established a model of bone marrow-derived murine macrophages (BMMs).
Analyzed M-CSF-induced MAPKs activation using Western blotting.
Examined macrophage development markers (CD14, F4/80) via FACS analysis.

Main Results:

M-CSF activated ERK, JNK, and p38 in both mature and immature macrophages.
cAMP inhibited M-CSF-induced MAPKs activation in a time-dependent manner.
Pretreatment with cAMP impaired macrophage development, affecting CD14 and F4/80 expression.

Conclusions:

cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.
These findings highlight a novel regulatory mechanism in macrophage differentiation.