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Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
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Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
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Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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ER Retrieval Pathway

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Related Experiment Video

Updated: Jul 5, 2026

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

LRRK2 regulates synaptic vesicle endocytosis.

Narae Shin1, Hyerhan Jeong, Jungsun Kwon

  • 1Department of Life Science, GIST, Buk-gu, Gwangju, South Korea.

Experimental Cell Research
|May 1, 2008
PubMed
Summary

Leucine-rich repeat kinase 2 (LRRK2) interacts with Rab5b, impacting synaptic vesicle endocytosis. This discovery offers new insights into Parkinson's disease (PD) mechanisms and potential therapeutic targets.

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Last Updated: Jul 5, 2026

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07:30

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Published on: September 4, 2017

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Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission F&#246;rster Resonance Energy Transfer (SE-FRET)
08:54

Monitoring Leucine-Rich Repeat Containing 8 Channel (LRRC8/VRAC) Activity Using Sensitized-Emission Förster Resonance Energy Transfer (SE-FRET)

Published on: August 9, 2024

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Leucine-rich repeat kinase 2 (LRRK2) is linked to Parkinson's disease (PD) pathogenesis.
  • The precise physiological roles of LRRK2 remain largely undefined.

Purpose of the Study:

  • To identify proteins interacting with LRRK2.
  • To elucidate the role of LRRK2 in synaptic function.

Main Methods:

  • Yeast two-hybrid screening to identify LRRK2-interacting proteins.
  • GST pull-down and co-immunoprecipitation assays to confirm interactions.
  • Subcellular fractionation, immunocytochemistry, and primary neuronal cell culture to assess protein localization and function.

Main Results:

  • Rab5b was identified as a novel LRRK2-interacting protein.
  • LRRK2 and Rab5b were found to co-localize in synaptic vesicles.
  • Altered LRRK2 expression impaired synaptic vesicle endocytosis, a defect rescued by functional Rab5b.

Conclusions:

  • LRRK2 interacts with Rab5b and plays a crucial role in synaptic vesicle endocytosis.
  • This interaction suggests a novel mechanism for LRRK2 in regulating synaptic function relevant to Parkinson's disease.