Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Osteoclasts in Bone Remodeling

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...

Bone Remodeling

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

Bone Formation by Endochondral Ossification

Bone Formation by Endochondral Ossification

Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A NOTCH2 pathogenic variant alters the osteoblast and vascular cell transcriptome of mouse femurs.

Bone·2026

Same author

Inactivation of Hes1 in Skeletal Undifferentiated Cells Increases Bone Volume.

Endocrinology·2026

Same author

A novel variant of NOTCH2 causes skeletal fragility.

Bone·2025

Same author

Role of Notch signaling in corticalization.

Journal of endocrinological investigation·2025

Same author

NOTCH2 disrupts the synovial fibroblast identity and the inflammatory response of epiphyseal chondrocytes.

The Journal of biological chemistry·2025

Same author

A NOTCH3 pathogenic variant influences osteogenesis and can be targeted by antisense oligonucleotides in induced pluripotent stem cells.

PloS one·2025

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Notch signaling in osteoblasts.

Ernesto Canalis¹

¹Department of Research, Saint Francis Hospital and Medical Center, 114 Woodland Street, Hartford, CT 06105, USA. ecanalis@stfranciscare.org

Science Signaling

|May 1, 2008

Summary

Notch signaling suppresses osteoblast and osteoclast differentiation, playing a key role in regulating bone remodeling. This discovery highlights Notch

Area of Science:

Cell biology
Developmental biology
Biochemistry

Background:

Bone remodeling is a continuous process involving osteoblasts (bone formation) and osteoclasts (bone resorption).
Notch proteins are transmembrane receptors crucial for cell fate determination.
Understanding the regulation of bone remodeling is vital for treating bone diseases.

Purpose of the Study:

To investigate the role of Notch signaling in osteoblast and osteoclast differentiation.
To elucidate the impact of Notch signaling on bone remodeling during development and postnatally.

Main Methods:

Utilizing gain-of-function and loss-of-function experiments.
Analyzing the effects on osteoblast and osteoclast differentiation pathways.
Examining Notch signaling in both developmental and postnatal bone contexts.

More Related Videos

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Related Experiment Videos

Last Updated: Jul 5, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

Main Results:

Notch signaling was found to suppress osteoblast differentiation.
Notch signaling was also found to suppress osteoclast differentiation.
These suppressive effects were observed during both bone development and in postnatal bone.

Conclusions:

Notch signaling plays a critical inhibitory role in regulating bone remodeling.
Targeting Notch signaling could offer new therapeutic strategies for bone disorders.
The findings establish Notch signaling as a key regulator in bone homeostasis.