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Probing the dynamics of protein-protein interactions at neuronal contacts by optical imaging

Olivier Thoumine1, Helge Ewers, Martin Heine

  • 1CNRS UMR 5091, Institut Magendie, Université Bordeaux 2, 33077 Bordeaux, France. othoumin@u-bordeaux2.fr

Chemical Reviews
|May 2, 2008
PubMed
Abstract

No abstract available in PubMed .

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Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...