Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
10:05

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Published on: May 23, 2020

Mouse embryonic stem cell-derived spheres with distinct neurogenic potentials.

Annalena Moliner1, Patrik Enfors, Carlos F Ibáñez

  • 1Department of Neuroscience, Karolinska Institute, 17177 Stockholm, Sweden.

Stem Cells and Development
|May 2, 2008
PubMed
Summary

Mouse embryonic stem cells form spheres that gain neuronal differentiation potential over time. This neurogenic potential is linked to metabolic changes and specific gene expression shifts during sphere development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Palmitoylation of death receptor p75<sup>NTR</sup> contributes to Alzheimer's disease progression by regulating APP trafficking and degradation.

Alzheimer's research & therapy·2026
Same author

Gpc3 selectively suppresses subcutaneous adipogenesis in diet-induced obesity.

PLoS biology·2026
Same author

Amelioration of Symptomatic Alzheimer's Disease after Selective Impairment of p75<sup>NTR</sup> Function in Adult Forebrain Excitatory Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Impaired migration and lung invasion of human melanoma by a novel small molecule targeting the transmembrane domain of death receptor p75<sup>NTR</sup>.

EMBO molecular medicine·2025
Same author

GDNF receptor GFRα1 is necessary for the maintenance of dopaminergic neurons in the adult substantia nigra.

PloS one·2025
Same author

A single-cell transcriptomic atlas of all cell types in the brain of 5xFAD Alzheimer mice in response to dietary inulin supplementation.

BMC biology·2025

Area of Science:

  • Stem Cell Biology
  • Developmental Neuroscience
  • Molecular Biology

Background:

  • Mouse embryonic stem cells (ESCs) cultured in suspension with LIF and bFGF form pluripotent spheres.
  • These spheres exhibit increasing neuronal differentiation competence with prolonged culture time.

Purpose of the Study:

  • To investigate the transcriptional changes associated with the acquisition of neurogenic potential in ESC-derived spheres.
  • To identify molecular mechanisms underlying the bias towards neuronal differentiation in ESCs.

Main Methods:

  • Gene expression profiling using oligonucleotide microarrays.
  • Quantitative real-time PCR for differential gene expression verification.
  • Gene Set Enrichment Analysis (GSEA) to identify enriched pathways.

More Related Videos

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
11:54

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Published on: January 11, 2011

Isolation and Culture of Embryonic Mouse Neural Stem Cells
09:04

Isolation and Culture of Embryonic Mouse Neural Stem Cells

Published on: November 11, 2018

Related Experiment Videos

Last Updated: Jul 5, 2026

Isolation and Expansion of Neurospheres from Postnatal (P1&#8722;3) Mouse Neurogenic Niches
10:05

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Published on: May 23, 2020

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay
11:54

Establishing Embryonic Mouse Neural Stem Cell Culture Using the Neurosphere Assay

Published on: January 11, 2011

Isolation and Culture of Embryonic Mouse Neural Stem Cells
09:04

Isolation and Culture of Embryonic Mouse Neural Stem Cells

Published on: November 11, 2018

Main Results:

  • Sphere development involved down-regulation of some ESC genes and up-regulation of novel genes over one week.
  • Late-stage spheres (8-day-old) showed significant neuronal differentiation, unlike early-stage spheres (3-day-old).
  • GSEA revealed correlation of neurogenic potential with mitochondrial function, metabolism, oxidative stress, and hypoxia pathways, alongside down-regulation of RNA transcription and proteasome pathways.

Conclusions:

  • ESC-derived spheres acquire a reversible proto-neurogenic state during development.
  • Cell-cell contact and paracrine interactions within the sphere niche influence metabolic state.
  • Metabolic state differences are crucial for biasing early ESC differentiation towards a neuronal phenotype.