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MMP-7 (-181A>G) promoter polymorphisms and risk for cervical cancer
HariOm Singh1, Meenu Jain, Balraj Mittal
1Department of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, India.
Objectives:
Matrix metalloproteinase (MMP-7) is a secreted matrilysin, which contributes to tumor progression through breakdown of basement membranes and angiogenesis. Therefore, we aimed to investigate the association of MMP-7 -181A>G gene polymorphism with risk of cervical cancer.
Methods:
In the present case control study, we enrolled a total of 150 cervical cancer patients confirmed by histopathology and 162 unrelated healthy individuals. Polymorphism for MMP-7 gene (-181A>G) was genotyped by polymerase chain reaction and restriction enzyme length polymorphism.
Results:
Frequency of MMP-7 -181GG genotype and -181G allele differed significantly between patients with cervical cancer (29.3%) and healthy individuals (19.4%) (P=0.041; OR(GG)=1.94 and P=0.048; OR(G)=1.94). Individuals with MMP-7 -181GG genotype were at higher risk of stage II of cervical cancer with borderline significance (P=0.05, OR=2.78; 95%CI: 0.89-8.69). However, interaction of MMP-7 -181AG and GG genotypes with tobacco usage did not modulate the cervical cancer risk significantly (OR 2.21, 95%CI=0.59-8.1 and OR 3.17, 95%CI=0.64-15.7).
Conclusion:
Individuals with MMP-7 -181GG genotype were at significantly higher risk of cervical cancer.
Insights
The MMP-7 -181GG genotype is linked to a higher risk of cervical cancer. This genetic variation may influence cancer development, warranting further investigation into its role in disease progression.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Matrix metalloproteinase-7 (MMP-7) is a key enzyme implicated in tumor progression.
- MMP-7 facilitates tumor growth by degrading basement membranes and promoting angiogenesis.
- Genetic variations in MMP-7 may influence an individual's susceptibility to cervical cancer.
Purpose of the Study:
- To investigate the association between the MMP-7 -181A>G gene polymorphism and the risk of developing cervical cancer.
- To determine if specific genotypes of MMP-7 are correlated with increased cervical cancer risk.
Main Methods:
- A case-control study was conducted with 150 cervical cancer patients and 162 healthy controls.
- Genotyping for the MMP-7 -181A>G polymorphism was performed using polymerase chain reaction and restriction enzyme length polymorphism.
Main Results:
- The frequency of the MMP-7 -181GG genotype and the -181G allele was significantly higher in cervical cancer patients compared to healthy individuals (P=0.041).
- Individuals with the MMP-7 -181GG genotype showed a trend towards higher risk for stage II cervical cancer (P=0.05).
- Interactions between MMP-7 genotypes and tobacco use did not significantly alter cervical cancer risk.
Conclusions:
- The MMP-7 -181GG genotype is associated with a significantly increased risk of cervical cancer.
- This genetic polymorphism may serve as a potential biomarker for cervical cancer risk assessment.
