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A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Occult hepatitis C virus infection revisited with ultrasensitive real-time PCR assay
Philippe Halfon1, Marc Bourlière, Denis Ouzan
1Laboratoire Alphabio, 23 rue de Friedland, Hôpital Ambroise Paré, 13006 Marseille, France. philippe.halfon@alphabio.fr
Abstract:
Occult hepatitis C infection is regarded as a new entity that should be considered when diagnosing patients with a liver disease of unknown origin. Using an ultrasensitive real-time PCR assay, we demonstrated that occult hepatitis C virus (HCV) infection cannot be found in peripheral blood mononuclear cells of patients with cryptogenic liver diseases, HCV--associated systemic vasculitis, or connective tissue diseases. The significance of such occult infection must be elucidated.
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