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Transgenic Organisms

Transgenic Organisms

Cloning of Dolly the Sheep

Cloning of Dolly the Sheep

The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...

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Updated: Jul 5, 2026

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

Published on: February 6, 2012

Transgenic sheep designed for transplantation studies.

William A Ritchie¹, Tim King, Claire Neil

¹Roslin Institute, Animal Services Department, Roslin, Midlothian, UK.

Molecular Reproduction and Development

|May 2, 2008

Summary

This summary is machine-generated.

Researchers created genetically modified (transgenic) animals using lentiviral vectors and embryo splitting. This technique produces identical twins, one of which is transgenic, paving the way for non-immunosuppressive transplantation.

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Last Updated: Jul 5, 2026

Germ Cell Transplantation and Testis Tissue Xenografting in Mice

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Published on: February 6, 2012

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Published on: September 30, 2010

Area of Science:

* Animal biotechnology
* Gene delivery systems
* Reproductive technologies

Background:

* Lentiviral vectors offer efficient transgene delivery to the animal germline.
* Producing genetically identical animals with distinct genetic modifications presents a challenge.

Purpose of the Study:

* To develop a method for generating monozygotic twin animals where only one twin is genetically modified.
* To explore the potential of this method for future applications in transplantation research.

Main Methods:

* Utilized lentiviral vectors for efficient germline gene transfer.
* Employed embryo splitting techniques to create monozygotic twins.
* Combined lentiviral transduction with embryo splitting to produce chimeric or selectively modified twins.

Main Results:

* Successfully produced monozygotic twins, with one twin confirmed as transgenic.
* Demonstrated the feasibility of generating genetically distinct, yet identical, twin animals.

Conclusions:

* The combined approach of lentiviral vector delivery and embryo splitting is effective for generating specific transgenic outcomes in monozygotic twins.
* This methodology holds promise for creating animal models for transplantation studies, potentially eliminating the need for immunosuppression due to genetic identity.