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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 5, 2026

Effective Detection of Hoechst Side Population Cells by Flow Cytometry
06:31

Effective Detection of Hoechst Side Population Cells by Flow Cytometry

Published on: August 23, 2024

Solid state yellow and orange lasers for flow cytometry.

Veena Kapoor1, Vladimir Karpov, Claudette Linton

  • 1Experimental Transplantation and Immunology Branch, NCI-NIH, Bethesda, Maryland 20892, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|May 2, 2008
PubMed
Summary
This summary is machine-generated.

New yellow and orange solid-state lasers now enable broader fluorochrome excitation in flow cytometry. This technology expands options for researchers needing specific wavelengths for advanced cellular analysis.

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Last Updated: Jul 5, 2026

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Area of Science:

  • Biotechnology
  • Optical Engineering

Background:

  • Flow cytometry commonly uses diode and DPSS lasers for fluorochrome excitation.
  • Limited practical yellow or orange laser sources hindered flow cytometry instrumentation.

Purpose of the Study:

  • Evaluate new solid-state laser systems (570-600 nm) for flow cytometry.
  • Assess their compatibility with existing flow cytometry platforms.

Main Methods:

  • Integrated DPSS lasers (580, 589, 592 nm) into BD LSR II and FACSVantage DiVa systems.
  • Excited fluorochromes like Texas Red, APC, HcRed, and Katushka.

Main Results:

  • Successfully integrated yellow and orange laser sources into flow cytometry platforms.
  • 592 nm laser was ideal for Texas Red; comparable excitation for APC/tandems vs. red lasers.
  • Optimal excitation for HcRed and Katushka achieved with yellow/orange lasers.

Conclusions:

  • Practical yellow and orange laser sources are now available for flow cytometry.
  • This technology addresses a critical need for visible light excitation across diverse fluorochromes.