Related Experiment Video
Updated: Jul 5, 2026

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Published on: October 22, 2012
In vivo hierarchy of individual T-cell epitope-specific helper T-cell subset against an intracellular bacterium
Toshi Nagata1, Taiki Aoshi, Masato Uchijima
1Department of Health Science, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan. tnagata@hama-med.ac.jp
Abstract:
Cellular immunity is indispensable for efficient protection against intracellular bacterial infection. CD4+ and CD8+ T cells specific for a variety of antigenic peptides derived from particular bacteria are induced after the infection. T cells recognizing different antigenic peptides have been speculated to have different functions in terms of the protective immunity. We here induced individual CD4+ T cells specific for each antigenic peptide derived from Listeria monocytogenes independently with DNA vaccines using gene gun bombardment and compared the CD4+ T-cell populations for their ability on the specific protective immunity against lethal listerial challenge and analyzed their characteristics.
Related Concept Videos
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
Cell-mediated Immune Responses
T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

