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Updated: Jul 5, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Cryobacterium mesophilum sp. nov., a novel mesophilic bacterium
Syed G Dastager1, Jae-Chan Lee, Yoon-Jung Ju
1Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea.
Abstract:
A novel mesophilic, Gram-positive, cream- to pale yellow-pigmented, aerobic bacterium, designated MSL-15T, was isolated from a soil sample collected from Bigeum Island, South Korea. The cells were catalase-positive, motile and irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain MSL-15T grew well between 20 and 28 degrees C, with optimum growth occurring at 25-28 degrees C. The major fatty acids were anteiso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C15 : 0. The genomic DNA G+C content was 70.0 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MSL-15T was associated with the genus Cryobacterium and exhibited 96.6 % sequence similarity to Cryobacterium psychrotolerans JCM 13925T and 94.8 % similarity to Cryobacterium psychrophilum JCM 1463T. Phenotypic, chemotaxonomic and phylogenetic data revealed that strain MSL-15T represents a novel species within the genus Cryobacterium, for which the name Cryobacterium mesophilum is proposed. The type strain is MSL-15T (=DSM 19267T=KCTC 19270T).
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