Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters

Pharmacogenetics of Drug Transporters: P-Glycoprotein and Solute Carrier Transporters

The pharmacogenetics of drug transporters is increasingly recognized as a critical factor influencing interindividual variability in drug absorption, distribution, and elimination. These membrane-bound proteins regulate drugs' movement across cellular barriers by actively pumping them out (efflux) or facilitating their uptake (influx). Among the major transporter families, ATP-binding cassette (ABC) and solute carrier (SLC) transporters play particularly prominent roles. Genetic polymorphisms...

ABC Transporters: Exporter

ABC Transporters: Exporter

ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...

Allosteric Proteins-ATCase

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...

ABC Transporters: Importer

ABC Transporters: Importer

ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Membrane curvature elastic stress triggers recruitment of PML-II onto the inner nuclear membrane.

Molecular biology of the cell·2025

Same author

Neurodevelopmental disease-causing variants in choline kinase CHKA gene couple phosphatidylcholine synthesis to oxidative stress damage and disease etiology.

The Journal of biological chemistry·2025

Same author

Primary envelopment of Kaposi's sarcoma-associated herpesvirus at the nucleoplasmic reticulum.

Journal of virology·2025

Same author

The proximity interactome of PML isoforms I and II under fatty acid stress.

FEBS letters·2024

Same author

Mechanisms for assembly of the nucleoplasmic reticulum.

Cellular and molecular life sciences : CMLS·2024

Same author

Nuclear lipid droplets in Caco2 cells originate from nascent precursors and in situ at the nuclear envelope.

Journal of lipid research·2024

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

OSBP negatively regulates ABCA1 protein stability.

Kristin Bowden¹, Neale D Ridgway

¹Department of Pediatrics and Biochemistry and Molecular Biology, Atlantic Research Centre, Dalhousie University, Halifax, NS, Canada.

The Journal of Biological Chemistry

|May 3, 2008

Summary

Oxysterol-binding protein (OSBP) negatively regulates liver X receptor (LXR) activity by decreasing ATP-binding cassette transporter A1 (ABCA1) protein levels, impacting cholesterol efflux pathways.

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Liver X receptors (LXR) activate genes for cholesterol efflux, like ABCA1.
Cytoplasmic sterol-binding proteins may influence this pathway.
Oxysterol-binding protein (OSBP) integrates sterol sensing with lipid synthesis and signaling.

Purpose of the Study:

Investigate OSBP's role in LXR regulation.
Determine OSBP's effect on ABCA1 expression and activity.

Main Methods:

RNA interference to suppress OSBP in Chinese hamster ovary cells and J774 macrophages.
Assessed ABCA1 protein and mRNA levels, cholesterol efflux, and LXR activity.
Investigated ABCA1 protein half-life and OSBP's sterol-binding domain activity.

More Related Videos

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Published on: August 25, 2023

Related Experiment Videos

Last Updated: Jul 5, 2026

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Analysis of SCAP N-glycosylation and Trafficking in Human Cells

Published on: November 8, 2016

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Published on: August 25, 2023

Main Results:

OSBP suppression increased ABCA1 protein and cholesterol efflux, independent of ABCA1 mRNA levels.
OSBP depletion extended ABCA1 protein half-life threefold.
OSBP's sterol-binding domain, not its interaction with organelles, mediated ABCA1 protein reduction.

Conclusions:

OSBP opposes LXR activity by destabilizing ABCA1 protein via its sterol-binding domain.
This mechanism impacts cellular cholesterol efflux regulation.