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Related Concept Videos

Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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Related Experiment Video

Updated: Jul 5, 2026

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
06:51

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Published on: May 6, 2020

Sequence-specific DNA cleavage mediated by bipyridine polyamide conjugates.

Philippe Simon1, Fabio Cannata, Loïc Perrouault

  • 1CNRS, UMR5153, Paris, F-75005, France.

Nucleic Acids Research
|May 3, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed novel bipyridine-polyamide molecules that precisely target and break specific DNA sequences in cells. This breakthrough offers new possibilities for genome editing and DNA repair research.

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Last Updated: Jul 5, 2026

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05:32

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

  • Molecular Biology
  • Chemical Biology
  • Genetics

Background:

  • Designing molecules to interact with specific DNA sequences is crucial for genome manipulation.
  • Polyamides can be engineered to recognize DNA sequences, but require conjugation to effectors for DNA damage.

Purpose of the Study:

  • To synthesize and evaluate bipyridine-polyamide conjugates for sequence-specific DNA cleavage in vitro and in cells.
  • To assess the DNA binding affinity and cell permeability of these novel conjugates.

Main Methods:

  • Synthesis of bipyridine-polyamide compounds with varying linkage positions.
  • In vitro DNA cleavage assays to determine specificity and yield.
  • Cell culture experiments to assess intracellular activity and cell penetration.

Main Results:

  • One bipyridine-polyamide conjugate demonstrated high affinity for its DNA target.
  • This conjugate induced specific, high-yield DNA cleavage both in vitro and in cultured cells.
  • The compound exhibited cell-penetrating properties, functioning when added directly to cell media.

Conclusions:

  • Bipyridine-polyamide conjugates can be designed to induce sequence-specific DNA breaks.
  • This strategy enables targeted genome modification and facilitates studies on DNA repair mechanisms.
  • The cell-penetrating nature of the conjugate enhances its potential for in vivo applications.