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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

PeptideAtlas: a resource for target selection for emerging targeted proteomics workflows.

Eric W Deutsch1, Henry Lam, Ruedi Aebersold

  • 1Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103, USA. edeutsch@systemsbiology.org

EMBO Reports
|May 3, 2008
PubMed
Summary

The PeptideAtlas Project offers a public database and tools for quantitative proteomics. This enables accurate measurement of system components, advancing systems biology research through mass spectrometry.

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Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

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Last Updated: Jul 5, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Area of Science:

  • Proteomics
  • Systems Biology
  • Mass Spectrometry

Background:

  • Systems biology requires reliable, quantitative measurements of all system components.
  • Current proteomics methods often lack complete data for perturbations.
  • Advancements in targeted quantitative mass spectrometry are needed.

Purpose of the Study:

  • To introduce the PeptideAtlas Project as a platform for quantitative proteomics.
  • To demonstrate how PeptideAtlas facilitates the selection and validation of mass spectrometry targets.
  • To enable a new era of proteomics research.

Main Methods:

  • Development of the PeptideAtlas database, a publicly accessible repository of identified peptides.
  • Inclusion of software tools for building and utilizing the PeptideAtlas database.
  • Leveraging tandem mass spectrometry data from numerous proteomics studies.

Main Results:

  • PeptideAtlas provides a comprehensive collection of peptide identifications.
  • Associated software tools enable community use and database construction.
  • The project offers a unique resource for mass spectrometry target validation.

Conclusions:

  • The PeptideAtlas Project is crucial for advancing quantitative proteomics.
  • It provides a foundation for reliable, gap-free data in systems biology.
  • This platform is poised to drive the next revolution in proteomics research.