Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluid Movement Between Compartments01:18

Fluid Movement Between Compartments

The force applied by fluids against a surface, known as hydrostatic pressure, initiates the transfer of fluid among different compartments. Within our blood vessels, the blood's hydrostatic pressure is a result of the heart's pumping action. At the arteriolar end of capillaries, hydrostatic pressure (capillary blood pressure) exceeds the opposing colloid osmotic pressure created primarily by plasma proteins like albumin. This discrepancy in pressure propels plasma and nutrients from the...
Aquaporins01:25

Aquaporins

Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Osmosis00:47

Osmosis

Approximately 60% to 95% of the weight of living organisms is attributed to water. Therefore, maintaining appropriate water balance within cells is of paramount importance. Osmosis is the movement of water across a semipermeable membrane, such as a cell’s plasma membrane. In living organisms, water plays a crucial role as a solvent—a molecule that dissolves other molecules.Diffusion Versus OsmosisBoth diffusion and osmosis are types of passive transport—cellular transport that does not require...
Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model01:09

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model

Various dissolution theories provide insight into the factors that influence the dissolution rate. Danckwerts' Model suggests that turbulence, rather than a stagnant layer, characterizes the dissolution medium at the solid-liquid interface. In this model, the agitated solvent contains macroscopic packets that move to the interface via eddy currents, facilitating the absorption and delivery of the drug to the bulk solution. The regular replenishment of solvent packets maintains the concentration...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PEERing into the Future: Benchmarking the ANSTO Australian Synchrotron's Very-High-Energy Electron Linac for Ultra-High Dose-Rate, In Vivo FLASH Radiotherapy Research.

Cancers·2026
Same author

A fasting-mimicking environment enhances procaspase-activating compound 1 in 2D and 3D glioma cell models.

Cell cycle (Georgetown, Tex.)·2026
Same author

Ornithine lipids from <i>Akkermansia muciniphila</i> are dynamically modulated in colitis and shape macrophage inflammatory responses.

Gut microbes·2025
Same author

Self-assembled cell-scale containers made from DNA origami membranes.

Nature materials·2025
Same author

Point cloud dosimetry framework for preclinical microbeam radiation therapy.

Physica medica : PM : an international journal devoted to the applications of physics to medicine and biology : official journal of the Italian Association of Biomedical Physics (AIFB)·2025
Same author

Hierarchical Structural Organization in Bioinspired Peptide Coacervate Microdroplets.

ACS nano·2025

Related Experiment Video

Updated: Jul 5, 2026

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

Down to atomic-scale intracellular water dynamics.

Marion Jasnin1, Martine Moulin, Martina Moulin

  • 1Institut de Biologie Structurale, UMR 5075, CEA-CNRS-UJF, 41 rue Jules Horowitz, 38027 Grenoble, France.

EMBO Reports
|May 3, 2008
PubMed
Summary
This summary is machine-generated.

Water dynamics inside bacteria are similar to pure water, challenging long-held beliefs about macromolecular confinement. This study used neutron scattering to reveal water

More Related Videos

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Related Experiment Videos

Last Updated: Jul 5, 2026

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
10:02

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water
08:48

High-Resolution Neutron Spectroscopy to Study Picosecond-Nanosecond Dynamics of Proteins and Hydration Water

Published on: April 28, 2022

Area of Science:

  • Biophysics
  • Cell Biology
  • Physical Chemistry

Background:

  • Water is the intracellular matrix for biological interactions.
  • Understanding intracellular water dynamics is a longstanding scientific challenge.
  • Previous studies have debated the effects of macromolecular confinement on water behavior.

Purpose of the Study:

  • To measure water dynamics in vivo within the cytoplasm of Escherichia coli.
  • To investigate water motion across various timescales and length scales.
  • To challenge the prevailing notion that cellular confinement 'tames' water.

Main Methods:

  • Neutron scattering techniques.
  • Isotope labeling for enhanced signal detection.
  • In vivo measurements within living bacterial cells (Escherichia coli).

Main Results:

  • Water diffusion within E. coli cytoplasm is comparable to pure water.
  • Observed water dynamics span from bulk-like to interfacial behaviors.
  • Atomic-level insights into water motion under physiological conditions were obtained.

Conclusions:

  • Intracellular water diffusion is not significantly hindered by macromolecular confinement.
  • Bacterial water exhibits properties similar to bulk water at physiological temperatures.
  • Challenges the established paradigm of 'tamed' intracellular water.