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Updated: Jul 5, 2026
07:07
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Published on: July 19, 2019
DigesTip: a new device for a rapid and efficient in-solution protein digestion
Roberto Marangoni1, Riccardo Chiarini, Giorgio Iannone
1ProteoGen Bio S.r.l., Pontedera, Pisa, Italy. marangon@di.unipi.it
Proteomics
|May 3, 2008
Summary
DigesTip, a novel pipette tip, enables rapid, in-solution protein digestion using immobilized enzymes. This technology significantly reduces digestion time and allows for analysis of low protein concentrations, improving mass spectrometry results.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Proteomics
Background:
- Traditional in-solution protein digestion can be time-consuming and requires specific sample concentrations.
- Enzyme immobilization on solid supports is an area of interest for improving digestion efficiency.
Purpose of the Study:
- To introduce DigesTip, a new device for rapid in-solution protein digestion.
- To evaluate the performance of DigesTip in terms of digestion time and sample concentration compatibility.
Main Methods:
- DigesTip utilizes a patent-pending technology to immobilize enzymes, such as trypsin, on a solid surface within a standard pipette tip.
- The device is compatible with both manual and robotic liquid handling systems.
Main Results:
- DigesTip achieves protein digestion in as little as 1 minute.
- The device is effective with low protein sample concentrations as low as 5 microg/mL.
- DigesTip produces a clear signal in mass spectrometry (MS) measurements.
- The use of DigesTip eliminates several traditional preparative steps.
Conclusions:
- DigesTip offers a significant advancement in protein digestion technology, enabling faster and more sensitive proteomic analyses.
- The device's ease of use and compatibility with automation make it a valuable tool for research and diagnostics.
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