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Updated: Jul 5, 2026
Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
Identification of up-regulated genes in malignant glioma with subtraction hybridization: preliminary screening
1Department of Neurosurgery, Ewha Womans University Mok-Dong Hospital, 911-1 Mok-dong, Yangchun-gu, Seoul 158-710, Korea. yongcho@ewha.ac.kr
Purpose:
This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization.
Materials And Methods:
Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank database.
Results:
Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line.
Conclusion:
Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
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