Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of the Lymphatic System01:15

Development of the Lymphatic System

The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Production of Formed Elements01:34

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Development of Blood Vessels01:07

Development of Blood Vessels

The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of cardiac regeneration capacity between zebrafish and medaka reveals a regenerative response in both teleost species.

NPJ Regenerative medicine·2026
Same author

Mitochondria directly interact with the nuclear pore complex.

Nature·2026
Same author

Pro-regenerative fingerprints identified in a sub-population of adult mouse cardiomyocytes by integrative single-cell proteomics.

Genome biology·2026
Same author

Titin cleavage in living cardiomyocytes induces sarcomere disassembly but does not trigger cell proliferation.

The Journal of biological chemistry·2026
Same author

In Science Journals.

Science (New York, N.Y.)·2026
Same author

Chromatin spatial analysis by METALoci unveils sex-determining 3D regulatory hubs.

Nature structural & molecular biology·2026

Related Experiment Video

Updated: Jul 5, 2026

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
07:30

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Published on: June 28, 2024

The dynamics of spleen morphogenesis.

Sally F Burn1, Marit J Boot, Carlo de Angelis

  • 1Medical and Developmental Genetics Section, MRC Human Genetics Unit, Edinburgh, EH4 2XU, UK.

Developmental Biology
|May 3, 2008
PubMed
Summary

Early spleen development in mammals involves specific cell origins and migration patterns. Researchers identified a conserved regulatory element guiding Nkx2-5 gene expression and pinpointed the anterior stomach

More Related Videos

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
08:04

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

Published on: June 11, 2019

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
11:36

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Published on: January 14, 2012

Related Experiment Videos

Last Updated: Jul 5, 2026

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
07:30

Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation

Published on: June 28, 2024

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity
08:04

A Mouse Model of Vascularized Heterotopic Spleen Transplantation for Studying Spleen Cell Biology and Transplant Immunity

Published on: June 11, 2019

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
11:36

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow

Published on: January 14, 2012

Area of Science:

  • Developmental Biology
  • Immunology
  • Hematology

Background:

  • The mammalian spleen is crucial for immunity and blood cell formation.
  • Early embryonic development of the spleen remains poorly understood.
  • Key cellular origins and migratory pathways are yet to be elucidated.

Purpose of the Study:

  • To investigate the cellular origins of splenic mesenchyme during embryonic development.
  • To understand the positional guidance mechanisms directing spleen precursor cells.
  • To identify regulatory elements controlling early spleen development.

Main Methods:

  • Utilized a transgenic mouse model with a reporter gene driven by a conserved Nkx2-5 regulatory element.
  • Employed organ culture systems to track spleen precursor cell migration.
  • Used a conditional Cre recombinase mouse line for lineage tracing of early precursor populations.

Main Results:

  • Spleen morphogenesis initiates bilaterally posterior to the stomach, with precursors migrating leftward.
  • Spleen precursor cells originate from posterior mesenchyme, distinct from stomach mesenchyme.
  • The anterior stomach plays a guidance role in directing splenic cell migration.

Conclusions:

  • The study elucidates the origin and migration of spleen precursor cells during mammalian embryonic development.
  • A conserved regulatory element controlling Nkx2-5 is vital for early spleen morphogenesis.
  • The anterior stomach actively guides spleen precursor cells to their final location.