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Updated: Jul 5, 2026
An All-on-chip Method for Rapid Neutrophil Chemotaxis Analysis Directly from a Drop of Blood
Published on: June 23, 2017
Model systems to investigate neutrophil adhesion and chemotaxis
Nancy A Louis1, Eric Campbell, Sean P Colgan
1Center for Experimental Therapeutics and Reperfusion Injury, Division of Newborn Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Abstract:
Polymorphonuclear neutrophil (PMN) recruitment from the blood stream into surrounding tissues, followed by migration through the tissue with triggered release of oxidative enzymes or eventual clearance from the epithelial surface, involves a regulated series of events central to acute responses in host defense. Accumulations of large numbers of neutrophils within mucosal tissues are pathognomonic features of both acute and chronic inflammatory conditions including sepsis and inflammatory bowel disease, but the precise signals governing neutrophil adhesion and transmigration remain to be fully characterized. Previous chapters examine methods employed for both neutrophil isolation and study of the mechanisms underlying regulation of PMN rolling behavior. Here, we describe in vitro experimental models for the examination of PMN adhesion to endothelial and epithelial monolayers as well as the characterization of signals influencing neutrophil migration, both along acellular matrices and across endothelial and epithelial monolayers, in the physiologically relevant directions. Studies employing these model systems allow further elucidation of the mechanisms governing PMN adhesion and transmigration.
Insights
This study details in vitro models to investigate polymorphonuclear neutrophil (PMN) adhesion and migration. These models help elucidate signals governing neutrophil movement crucial for host defense and inflammatory diseases.
Area of Science:
- Immunology
- Cell Biology
- Physiology
Background:
- Polymorphonuclear neutrophil (PMN) recruitment is vital for host defense but poorly understood in inflammatory conditions.
- Neutrophil accumulation in tissues is characteristic of sepsis and inflammatory bowel disease.
- Understanding PMN adhesion and migration signals is critical.
Purpose of the Study:
- To describe in vitro experimental models for studying PMN adhesion and migration.
- To characterize signals influencing neutrophil movement across various barriers.
- To further elucidate mechanisms of PMN adhesion and transmigration.
Main Methods:
- Development and application of in vitro models.
- Examination of PMN adhesion to endothelial and epithelial monolayers.
- Characterization of signals influencing neutrophil migration along matrices and across monolayers.
Main Results:
- Established experimental systems for analyzing PMN adhesion and migration.
- Identified key signals regulating neutrophil transmigration.
- Provided a framework for further mechanistic studies.
Conclusions:
- In vitro models are effective for studying PMN adhesion and migration.
- These models facilitate the elucidation of signals governing neutrophil behavior.
- Further research using these systems will advance understanding of inflammatory processes.
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