Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

Genome-scale transcript analyses in human neutrophils.

Scott D Kobayashi1, Dan E Sturdevant, Frank R DeLeo

  • 1Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Staphylococcus aureus</i> transcriptomics and single-cell sequencing approaches.

Infection and immunity·2025
Same author

Evaluation of capsule polysaccharide (CPS)-specific antibodies for broad recognition of prominent multidrug-resistant <i>Klebsiella pneumoniae</i>.

Microbiology spectrum·2025
Same author

<i>Staphylococcus aureus</i> SaeR/S-regulated factors overcome human complement-mediated inhibition of aggregation to evade neutrophil killing.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Carbapenem-Resistant, Virulence Plasmid-Harboring Klebsiella pneumoniae, United States.

Emerging infectious diseases·2025
Same author

Insights into the molecular basis of reduced vancomycin susceptibility among three prominent <i>Staphylococcus aureus</i> clonal complexes.

Microbiology spectrum·2024
Same author

Zika virus diversity in mice is maintained during early vertical transmission from placenta to fetus, but reduced in fetal bodies and brains at late stages of infection.

PLoS neglected tropical diseases·2023

This study details a standardized method for analyzing gene expression in human neutrophils using oligonucleotide microarrays. These methods provide new insights into molecular processes resolving human infections.

Area of Science:

  • Molecular Biology
  • Immunology
  • Genomics

Background:

  • Transcriptome analyses have transformed biological and pathological understanding.
  • Neutrophil gene expression studies offer insights into infection resolution.

Purpose of the Study:

  • To present standardized methods for analyzing gene expression in human neutrophils.
  • To facilitate reproducible research in neutrophil transcriptomics.

Main Methods:

  • Isolation of high-quality RNA from human neutrophils.
  • Generation and labeling of complementary RNA (cRNA).
  • Affymetrix GeneChip hybridization and scanning.

Main Results:

  • The described methods utilize commercially available reagents and materials.

More Related Videos

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
11:03

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

Published on: June 18, 2016

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
11:21

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Published on: February 16, 2020

Related Experiment Videos

Last Updated: Jul 5, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro
11:03

High Throughput Measurement of Extracellular DNA Release and Quantitative NET Formation in Human Neutrophils In Vitro

Published on: June 18, 2016

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
11:21

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Published on: February 16, 2020

  • This approach allows for standardized evaluation of neutrophil transcript levels.

    • Conclusions:

    • The presented methods offer a standardized approach for human neutrophil gene expression analysis.
    • This standardization is crucial for advancing our understanding of infection resolution processes.