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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

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Related Experiment Video

Updated: Jul 5, 2026

Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

Digital imaging of stem cells by electron microscopy.

A Henry Sathananthan1, Stefania A Nottola

  • 1Monash Immunology & Stem Cell Laboratories, Monash University, Melbourne, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2008
PubMed
Summary

Scanning and transmission electron microscopy reveal detailed stem cell structures. These advanced imaging techniques help confirm stem cell identity and morphology during differentiation.

Area of Science:

  • Cell Biology
  • Microscopy Techniques

Background:

  • Characterizing stem cell fine structure is crucial for identity confirmation.
  • Supplementing phase-contrast and confocal microscopy with electron microscopy provides deeper insights.
  • Understanding stem cell morphology aids in comparing them with existing literature on cells and tissues.

Purpose of the Study:

  • To present basic techniques for scanning and transmission electron microscopy (SEM & TEM).
  • To apply these techniques for imaging embryonic and adult stem cells.
  • To visualize stem cell surface and internal morphology during differentiation.

Main Methods:

  • Utilizing scanning electron microscopy (SEM) for surface morphology.
  • Employing transmission electron microscopy (TEM) for internal ultrastructure.

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  • Preparing stem cell samples for high-resolution electron imaging.

    • Main Results:

    • Detailed images of stem cell surface features were obtained using SEM.
    • TEM revealed intricate internal structures of stem cells.
    • Electron microscopy provided complementary data to light microscopy techniques.

    Conclusions:

    • SEM and TEM are effective methods for characterizing stem cell fine structure.
    • These techniques enhance the identification and morphological analysis of stem cells.
    • Visualizing stem cells with electron microscopy aids in understanding their differentiation processes.