Jove
Visualize
Contact Us

Related Concept Videos

Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 3D Human Neuron-on-Chip Platform to Monitor Neuronal Injury Responses.

bioRxiv : the preprint server for biology·2026
Same author

Alkaline loading of extracellular vesicles produced from human neural stem cell-derived neurospheres enables CNS drug delivery.

Scientific reports·2026
Same author

Mitochondrial morphology in human fibroblasts and induced pluripotent stem cells in Leigh syndrome: A comparative analysis.

Physiological reports·2026
Same author

Serum Neurofilament Light Chain Predicts Stroke Outcome and is a Potential Marker for Treatment Effects of Neural Stem Cell-derived Extracellular Vesicles in a Rat Stroke Model.

Translational stroke research·2026
Same author

Disrupting Akt-Wnt/β-catenin signaling suppresses glioblastoma stem cell growth and tumor progression in immunocompetent mice.

Journal of neuro-oncology·2026
Same author

Investigating the impact of multinational collaborations on cultural understanding, health disparities, biomedical innovations, and professional development through project-based learning.

Journal of biological engineering·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 5, 2026

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
12:43

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Published on: December 6, 2013

Cell surface markers in human embryonic stem cells.

Raj R Rao1, Alison Venable Johnson, Steven L Stice

  • 1Department of Chemical and Life Science Engineering, Virginia Commonwealth University, Richmond, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 6, 2008
PubMed
Summary
This summary is machine-generated.

Researchers identified specific lectins as potential markers for human embryonic stem cells (hESCs). These lectins, similar to SSEA-4, can help classify pluripotent hESCs and differentiate them from other cell types.

More Related Videos

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
07:12

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

Published on: October 28, 2012

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Related Experiment Videos

Last Updated: Jul 5, 2026

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2
12:43

Enrichment and Purging of Human Embryonic Stem Cells by Detection of Cell Surface Antigens Using the Monoclonal Antibodies TG30 and GCTM-2

Published on: December 6, 2013

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures
07:12

Rapid Fibroblast Removal from High Density Human Embryonic Stem Cell Cultures

Published on: October 28, 2012

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Area of Science:

  • Stem Cell Biology
  • Glycobiology
  • Cell Surface Characterization

Background:

  • Human embryonic stem cells (hESCs) possess pluripotency, enabling differentiation into all body cell types.
  • Thorough characterization of hESCs is crucial before directed differentiation.
  • Cell surface glycoproteins and carbohydrates offer potential markers for hESC identification and differentiation state delineation.

Purpose of the Study:

  • To investigate novel pluripotent markers on hESCs using a panel of lectins.
  • To compare lectin binding patterns with the established pluripotent marker SSEA-4.
  • To explore the utility of lectins for classifying pluripotent hESCs and distinguishing differentiated cell types.

Main Methods:

  • Utilized a panel of 14 lectins with known carbohydrate specificities.
  • Employed flow cytometry for quantitative analysis of lectin binding to hESCs.
  • Applied immunocytochemistry to localize lectin binding within adherent hESC colonies.

Main Results:

  • Identified specific lectins exhibiting binding percentages and localization patterns analogous to SSEA-4.
  • Demonstrated that certain lectins are associated with the pluripotent state of hESCs.
  • Showcased lectin binding as a potential indicator of hESC pluripotency.

Conclusions:

  • Certain lectins can serve as reliable markers for the pluripotent state of hESCs.
  • Lectin-based analysis provides a method for systematic classification of pluripotent hESCs.
  • Glycan presentation changes during differentiation can be effectively monitored using lectins to distinguish cell types.