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Updated: Jul 5, 2026

Sexual Development and Ascospore Discharge in Fusarium graminearum
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Developmental changes in the(45)Ca (2+) uptake byTrichoderma viride mycelium.

M Simkovic1, R Ortega-Perez, G Turian

  • 1Department of Biochemistry and Microbiology, Slovak Technical University, Bratislava, Slovakia.

Folia Microbiologica
|April 1, 1997
PubMed
Summary
This summary is machine-generated.

Calcium-45 (45Ca2+) uptake by Trichoderma viride mycelium indicates biological age, peaking around 30 hours. Uptake declines with increased mycelial mass, suggesting age-related changes in nutrient absorption.

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Area of Science:

  • Mycology
  • Biochemistry
  • Cell Biology

Background:

  • Trichoderma viride is a filamentous fungus with significant ecological and industrial applications.
  • Understanding nutrient uptake dynamics is crucial for optimizing fungal growth and metabolic processes.
  • Calcium (Ca2+) plays vital roles in fungal cell wall structure, signaling, and development.

Purpose of the Study:

  • To investigate the kinetics of calcium-45 (45Ca2+) uptake by submerged Trichoderma viride mycelium.
  • To determine the relationship between 45Ca2+ uptake rate and the biological age of the fungal culture.
  • To compare 45Ca2+ uptake in submerged versus surface-cultivated mycelia and analyze its distribution during different growth phases.

Main Methods:

  • Submerged cultivation of Trichoderma viride.
  • Measurement of 45Ca2+ uptake over time at various culture ages.
  • Analysis of mycelial mass and its correlation with uptake rates.
  • Autoradiography to visualize 45Ca2+ distribution within fungal colonies.

Main Results:

  • 45Ca2+ uptake rate in submerged cultures increased from 6 h, peaking around 30 h, then decreased significantly with increasing mycelial mass.
  • Uptake rate could not be restored by medium replacement or dilution, indicating an age-dependent phenomenon.
  • Surface-cultivated mycelium showed time-proportional 45Ca2+ uptake.
  • Autoradiography revealed homogeneous 45Ca2+ distribution during vegetative growth, with massive accumulation in conidia during conidiation.

Conclusions:

  • The rate of 45Ca2+ uptake serves as a reliable indicator of the biological age of submerged Trichoderma viride cultures.
  • Fungal growth phase, specifically conidiation, influences calcium localization and accumulation within the mycelium.
  • Differential calcium uptake and distribution highlight distinct physiological states during fungal development.