Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methods for Assessing Scaffold Vascularization with Human Endothelial Cells.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

NOS3 regulates angiogenic potential of human induced pluripotent stem cell-derived endothelial cells.

Biochemistry and biophysics reports·2024
Same author

Hierarchically vascularized and suturable tissue constructs created through angiogenesis from tissue-engineered vascular grafts.

Acta biomaterialia·2024
Same author

Liver specification of human iPSC-derived endothelial cells transplanted into mouse liver.

JHEP reports : innovation in hepatology·2024
Same author

PI(4,5)P<sub>2</sub>-dependent regulation of endothelial tip cell specification contributes to angiogenesis.

Science advances·2023
Same author

Engineering transplantable human lymphatic and blood capillary networks in a porous scaffold.

Journal of tissue engineering·2023
Same journal

Photobiomodulation Targets Mitochondrial Homeostasis for Diabetic Wound Healing.

Tissue engineering. Part B, Reviews·2026
Same journal

Nanotechnology-Driven Noninvasive and Targeted Therapies for Anaplastic Thyroid Carcinoma.

Tissue engineering. Part B, Reviews·2026
Same journal

From Restoration to Regeneration: A Review of 3D Printing Strategies in Digital Dentistry.

Tissue engineering. Part B, Reviews·2026
Same journal

Molecular Pathogenesis of and Regenerative Strategies for Osteonecrosis of the Femoral Head.

Tissue engineering. Part B, Reviews·2026
Same journal

Regenerative Potential of Platelet-Rich Plasma-Loaded Scaffolds in Endometrial Damage: A Meta-Analysis.

Tissue engineering. Part B, Reviews·2026
Same journal

Enhancing Round Window Membrane Permeability for Inner Ear Drug Delivery: A Systematic Review.

Tissue engineering. Part B, Reviews·2026
See all related articles

Related Experiment Video

Updated: Jul 5, 2026

Micropatterning and Assembly of 3D Microvessels
13:05

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Engineering the microcirculation.

Zerina Lokmic1, Geraldine M Mitchell

  • 1Bernard O'Brien Institute of Microsurgery, Melbourne, Victoria, Australia.

Tissue Engineering. Part B, Reviews
|May 6, 2008
PubMed
Summary
This summary is machine-generated.

Ensuring blood supply for tissue-engineered constructs is crucial for survival. This review explores methods for creating vascular networks and addresses challenges in connecting them to host circulation and preventing regression.

More Related Videos

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
09:39

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Published on: May 19, 2016

Related Experiment Videos

Last Updated: Jul 5, 2026

Micropatterning and Assembly of 3D Microvessels
13:05

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip
10:55

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

Published on: October 21, 2013

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production
09:39

Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production

Published on: May 19, 2016

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Successful tissue engineering requires functional vascularization for nutrient and oxygen delivery.
  • Connecting engineered microvasculature to host circulation is a major hurdle for construct survival.
  • Challenges include vascular integration, volume expansion, and preventing regression.

Purpose of the Study:

  • To review current strategies for vascularizing tissue-engineered constructs.
  • To identify key components: cells, growth factors, delivery systems, and scaffolds.
  • To examine issues of vascular remodeling and regression.

Main Methods:

  • Overview of in vitro and in vivo vascularization techniques.
  • Analysis of cell types, growth factors, and delivery mechanisms.
  • Evaluation of scaffold properties influencing microcirculation formation.

Main Results:

  • Advances in forming microcirculatory networks for 3D constructs.
  • Identification of unresolved issues: host connection, volume expansion, and regression.
  • Insights into factors controlling vascular remodeling.

Conclusions:

  • Developing permanent microcirculatory networks necessitates optimized cells, growth factors, delivery, and scaffolds.
  • Addressing vascular integration and stability is critical for clinical translation.
  • Further research is needed to overcome vascular regression and remodeling challenges.