Related Experiment Video

Updated: Jul 5, 2026

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)
12:19

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

Published on: May 27, 2012

Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy

Guo Fu1, Feng Zhang, Li Cao

  • 1Institute of Neuroscience, Department of Neurobiology, Second Military Medical University, Shanghai 200433, China.

Biophysical Chemistry
|May 6, 2008
PubMed

Insights

Dok5 protein constitutively targets the plasma membrane, with its PH domain being crucial for this localization. Single-molecule analysis shows Dok5 also partitions into plasma membrane microdomains, offering insights into its cellular behavior.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • The Dok5 protein plays a role in cellular signaling pathways.
  • Understanding protein localization is key to deciphering cellular functions.
  • Plasma membrane microdomains are critical for organizing cellular processes.

Purpose of the Study:

  • To investigate the plasma membrane targeting of Dok5.
  • To determine the role of the PH domain in Dok5 localization.
  • To analyze the microdomain partitioning of Dok5 in living cells.

Main Methods:

  • Single-molecule fluorescence microscopy was employed.
  • Enhanced yellow fluorescent protein (eYFP)-tagged Dok5 and its variants were used.
  • Analysis of single-molecule trajectories in Chinese hamster ovary (CHO) cells.

Main Results:

  • Dok5 constitutively targets the plasma membrane.
  • The PH domain of Dok5 is essential for plasma membrane targeting.
  • Dok5 constitutively partitions into plasma membrane microdomains.

Conclusions:

  • Dok5 localization to the plasma membrane is constitutive and PH domain-dependent.
  • Dok5 exhibits constitutive partitioning into plasma membrane microdomains.
  • This study elucidates Dok5's membrane targeting and microdomain localization mechanisms.

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

Published on: February 17, 2023

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...