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Updated: Jul 5, 2026

Inner Mitochondrial Membrane Sensitivity to Na+ Reveals Partially Segmented Functional CoQ Pools
Published on: July 20, 2022
Mammalian complex I: a regulable and vulnerable pacemaker in mitochondrial respiratory function
Sergio Papa1, Domenico De Rasmo, Salvatore Scacco
1Department of Medical Biochemistry, Biology and Physics (DIBIFIM), University of Bari, Italy. papabchm@cimedoc.uniba.it
Abstract:
In this paper the regulatory features of complex I of mammalian and human mitochondria are reviewed. In a variety of mitotic cell-line cultures, activation in vivo of the cAMP cascade, or direct addition of cAMP, promotes the NADH-ubiquinone oxidoreductase activity of complex I and lower the cellular level of ROS. These effects of cAMP are found to be associated with PKA-mediated serine phosphorylation in the conserved C-terminus of the subunit of complex I encoded by the nuclear gene NDUFS4. PKA mediated phosphorylation of this Ser in the C-terminus of the protein promotes its mitochondrial import and maturation. Mass-spectrometry analysis of the phosphorylation pattern of complex I subunits is also reviewed.
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