Related Experiment Video
Updated: Jul 5, 2026
13:56
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Published on: July 18, 2013
Karyopherin beta3: a new cellular target for the HPV-16 E5 oncoprotein
Ewa Krawczyk1, John A Hanover, Richard Schlegel
1Department of Pathology, Georgetown University Medical School, 3900 Reservoir Road NW, Washington, DC 20057, USA.
Summary
High-risk human papillomaviruses (HPVs) cause cervical cancer. This study identifies karyopherin beta3 as a new cellular target interacting with the HPV-16 E5 protein, a key factor in cancer development.
Area of Science:
- Oncology
- Virology
- Cell Biology
Background:
- High-risk human papillomaviruses (HPVs) are linked to cervical cancer.
- HPV-16 causes over half of these cancers, with E6 and E7 oncoproteins being well-studied.
- Emerging evidence highlights the HPV-16 E5 protein (16E5) as a significant mediator of oncogenic transformation.
Purpose of the Study:
- To identify novel cellular targets interacting with the HPV-16 E5 protein.
- To investigate the role of 16E5 in cellular processes relevant to oncogenesis.
Main Methods:
- Co-immunoprecipitation assays were used to detect protein interactions.
- Experiments were conducted in COS cells and stable human ectocervical cell lines.
Main Results:
- A new cellular target, karyopherin beta3, was identified that binds to 16E5.
- Karyopherin beta3 is a nuclear import receptor involved in ribosomal protein import and the secretory pathway.
Conclusions:
- Karyopherin beta3 is a novel binding partner for the HPV-16 E5 protein.
- This interaction may contribute to the oncogenic mechanisms of HPV-16 by affecting cellular transport pathways.
Related Concept Videos
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
02:38Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:33Cancer-Critical Genes I: Proto-oncogenes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:03Rous Sarcoma Virus (RSV) and Cancer
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...