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Updated: Jul 5, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
Characterization of pore structure of chromatographic adsorbents employed in separation of monoclonal antibodies
Ivana Tatárová1, Michal Gramblicka, Monika Antosová
1Department of Chemical and Biochemical Engineering, Institute of Chemical and Environmental Engineering, Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinského 9, SK-812 37 Bratislava, Slovakia.
Abstract:
Structural properties of commercial chromatographic adsorbents designated for separation of monoclonal antibodies were investigated using size-exclusion techniques. A batch technique provided the specific pore volumes distributed among small, medium and large pores. Inverse size-exclusion chromatography yielded the partition coefficients of dextran solute probes in medium pores. These data were fitted with one- and two-parametric models corresponding to different pore-size distribution functions and the suitability of the individual models for the characterization of the examined materials was then assessed. The reliability of estimation of the parameters of log-normal distribution of cylindrical pores was investigated. Large uncertainties and a strong correlation of the mean pore diameter and width of distribution function were observed.
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