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Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system
Shinji Fukuda1, Yukie Sasaki, Masato Seno
1Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan. s-fukuda80723@pref.hiroshima.lg.jp
Applied and Environmental Microbiology
|May 6, 2008
Abstract:
We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.
