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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jul 5, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Taking microRNAs to heart.

Thomas E Callis1, Da-Zhi Wang

  • 1Carolina Cardiovascular Biology Center, University of North Carolina, Chapel Hill, NC 27599-7126, USA.

Trends in Molecular Medicine
|May 7, 2008
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) are small RNAs crucial for heart development and function. Their dysregulation is linked to heart disease, highlighting their potential as diagnostic markers and therapeutic targets.

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Related Experiment Videos

Last Updated: Jul 5, 2026

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Cardiovascular Science

Background:

  • MicroRNAs (miRNAs) are small, non-coding RNAs regulating gene expression post-transcriptionally.
  • miRNA dysregulation is associated with human heart disease.
  • miRNAs are essential for cardiac development and function, as shown in murine models.

Purpose of the Study:

  • To explore the roles of miRNAs in cardiac development and pathology.
  • To investigate the potential of miRNAs as biomarkers and therapeutic targets for cardiovascular disease.

Main Methods:

  • Review of genetic studies and experimental data on miRNA function in the heart.
  • Analysis of miRNA expression patterns in diseased hearts.

Main Results:

  • Specific miRNAs play distinct roles in cardiogenesis, hypertrophy, and electrical conduction.
  • Inhibiting miRNA maturation in murine hearts impairs cardiac development and function.

Conclusions:

  • miRNAs are critical regulators of cardiac biology.
  • Understanding miRNA mechanisms offers new insights into heart development and disease.
  • miRNAs represent promising diagnostic and therapeutic avenues for cardiovascular conditions.