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Related Concept Videos

Thermosensation01:43

Thermosensation

Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

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Related Experiment Video

Updated: Jul 5, 2026

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

Human lung epithelial cells express a functional cold-sensing TRPM8 variant.

Ashwini S Sabnis1, Mohammad Shadid, Garold S Yost

  • 1University of Utah, Department of Pharmacology and Toxicology, Salt Lake City, UT 84112, USA.

American Journal of Respiratory Cell and Molecular Biology
|May 7, 2008
PubMed
Summary
This summary is machine-generated.

A novel TRPM8 variant in lung cells responds to cold and menthol, releasing inflammatory cytokines. This may explain cold-induced asthma hypersensitivity.

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Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
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Published on: December 31, 2013

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Last Updated: Jul 5, 2026

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
12:09

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4

Published on: December 31, 2013

Area of Science:

  • Ion channel research
  • Respiratory cell biology
  • Molecular physiology

Background:

  • Transient receptor potential (TRP) channels, including TRPM8, detect temperature and chemical stimuli.
  • TRPM8 is known for sensing cold and menthol, mediating neuronal signals.
  • The role of TRPM8 in non-neuronal cells, like lung epithelial cells, remains largely uninvestigated.

Purpose of the Study:

  • To investigate the expression and function of a TRPM8 variant in human bronchial epithelial cells.
  • To determine the cellular localization and activation mechanisms of this TRPM8 variant.
  • To explore the functional consequences of TRPM8 activation in lung epithelial cells.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR), cloning, and immunohistochemistry were used to confirm TRPM8 variant expression.
  • Cellular responses were measured upon activation with menthol and cold (18°C).
  • TRPM8 antagonist (N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl)piperazine-1-carboxamide) and thapsigargin, along with shRNA, were used to assess TRPM8 function.

Main Results:

  • A truncated TRPM8 variant was identified and localized to endoplasmic reticulum membranes in lung epithelial cells.
  • Menthol and cold exposure activated the TRPM8 variant, leading to increased expression of inflammatory cytokines IL-6 and IL-8.
  • TRPM8 activation was inhibited by specific antagonists and shRNA suppression, confirming receptor specificity.

Conclusions:

  • A novel TRPM8 variant in human lung epithelial cells functions as a cold and menthol sensor.
  • Activation of this TRPM8 variant modulates inflammatory responses in the respiratory system.
  • This finding offers a potential mechanism underlying cold-induced hypersensitivity in asthma.