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Interaction between ceruloplasmin and Sendai virus envelope components. Note i. Gel-filtration of Tween
Virologie
|April 1, 1976
Abstract:
Sindai virus was disrupted by treatment with different concentrations of Tween 20 in an alkaline medium and the variations in infectivity, turbidity, hemagglutinin, neuraminidase and hemolytic activities were followed up. Gel-filtration of virus envelope fragments obtained by disruption with 1% Tween 20 demonstrated their heterogeneity as regards shape, size and biological activity. The elution type, the ratio between hemagglutinin and neuraminidase activities and the affinity for red blood cells of the envelope fragments depend on the detergent concentration used for virus disruption and on the presence or absence of detergent in the eluent.