Related Experiment Video
Updated: Jul 5, 2026
Evaluating the Immune Response of a Nanoemulsion Adjuvant Vaccine Against Methicillin-Resistant Staphylococcus aureus (MRSA) Infection
Published on: September 1, 2023
The effects of increasing sodium chloride concentration on Mycoplasma gallisepticum vaccine survival in solution
S A Leigh1, J D Evans, S L Branton
1USDA, ARS South Central Poultry Research Unit, Mississippi State, MS 39762, USA. sleigh@msa-msstate.ars.usda.gov
Abstract:
Lyophilized Mycoplasma gallisepticum (MG) vaccines are generally rehydrated and diluted with distilled or chlorine-free water as per manufacturer recommendations. However, as mycoplasma species lack a cell wall, this can lead to decreased viability of live vaccine during administration. The ability of phosphate-buffered saline (PBS) to prevent losses in live vaccine viability was examined. It was shown that a concentration of 1 x PBS prevented the two-fourfold decrease in MG viability seen when the vaccines were diluted with water alone.
Insights
Rehydrating lyophilized Mycoplasma gallisepticum (MG) vaccines with phosphate-buffered saline (PBS) instead of water significantly improves live vaccine viability. This simple change prevents substantial losses during administration, ensuring better vaccine efficacy.
Area of Science:
- Veterinary Microbiology
- Vaccine Technology
- Poultry Health
Background:
- Lyophilized Mycoplasma gallisepticum (MG) vaccines require rehydration before administration.
- Standard rehydration protocols often use distilled or chlorine-free water.
- Mycoplasma species' lack of a cell wall makes them susceptible to viability loss during dilution.
Purpose of the Study:
- To evaluate the efficacy of phosphate-buffered saline (PBS) in maintaining the viability of live Mycoplasma gallisepticum vaccines.
- To compare the protective effect of PBS against the viability loss observed with water dilution.
Main Methods:
- Lyophilized Mycoplasma gallisepticum vaccines were rehydrated and diluted.
- Diluents tested included distilled water and phosphate-buffered saline (PBS) at a 1x concentration.
- Vaccine viability was assessed after dilution in each medium.
Main Results:
- Dilution with water alone resulted in a two-to-fourfold decrease in Mycoplasma gallisepticum viability.
- Using 1x phosphate-buffered saline (PBS) prevented this significant loss in vaccine viability.
- PBS maintained higher live vaccine titers compared to water.
Conclusions:
- Phosphate-buffered saline (PBS) is a superior diluent for lyophilized Mycoplasma gallisepticum vaccines compared to water.
- The use of PBS enhances the viability of live MG vaccines, potentially improving efficacy.
- This finding offers a practical improvement for vaccine administration protocols in poultry.
