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Related Experiment Video

Updated: Jul 5, 2026

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
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Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

Protein-membrane interaction probed by single plasmonic nanoparticles.

Cristina L Baciu1, Jan Becker, Andreas Janshoff

  • 1Institute of Physical Chemistry, University of Mainz, Mainz, Germany.

Nano Letters
|May 8, 2008
PubMed
Summary

We developed a new sensor using gold nanorods to detect protein interactions with lipid bilayers. This platform enables precise analysis of biological membrane composition and protein binding events.

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Materials Science

Background:

  • Biological membranes are crucial for cellular functions.
  • Understanding protein-membrane interactions is vital for disease research.
  • Existing methods for analyzing these interactions can be limited in scope.

Purpose of the Study:

  • To develop a nanosized sensor platform for studying protein-membrane interactions.
  • To demonstrate the detection of streptavidin binding to biotinylated lipids on membrane-coated plasmonic particles.
  • To enable local analysis of protein interactions with phase-separated biological membranes.

Main Methods:

  • Fabrication of membrane-coated plasmonic nanoparticles.
  • Coating gold nanorods with lipid bilayers.

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
09:29

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

Related Experiment Videos

Last Updated: Jul 5, 2026

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers
09:33

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

Published on: March 21, 2025

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation
09:29

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

  • Utilizing fast single particle spectroscopy (fastSPS) for parallel particle analysis.
  • Monitoring spectral shifts to detect binding events.

    • Main Results:

    • Successfully demonstrated the coating of plasmonic particles with lipid bilayers.
    • Unequivocally detected streptavidin binding to biotinylated lipids on the membrane.
    • Achieved parallel detection of binding events on numerous particles using fastSPS.
    • Showcased the ability to analyze protein interactions as a function of membrane composition.

    Conclusions:

    • The developed sensor platform is effective for studying protein-membrane interactions at the nanoscale.
    • Fast single particle spectroscopy provides a powerful tool for analyzing binding kinetics and membrane composition.
    • This approach offers new possibilities for local analysis of biological membrane behavior.