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CD26 expression on CD4+T cells in patients with cutaneous leishmaniasis
R Jafari-Shakib1, S Ajdary, Z Mohtasham Amiri
1National Cell Bank of Iran, Pasteur Institute, Tehran, Iran.
Clinical and Experimental Immunology
|May 8, 2008
Summary
CD26 expression on T cells is elevated in both healing and non-healing cutaneous leishmaniasis (CL). However, CD26 is not a reliable surrogate marker for interferon-gamma production in CL patients.
Area of Science:
- Immunology
- Parasitology
- Dermatology
Background:
- Defining reliable surrogate markers for protection in human leishmaniasis remains a challenge.
- Understanding immune responses, including T helper 1 (Th1) and Th2 cytokines and CD26 expression, is crucial for evaluating leishmaniasis pathogenesis.
Purpose of the Study:
- To assess T helper 1 (Th1) and Th2 cytokine profiles and CD26 expression on CD4(+) T cells in patients with healing or non-healing cutaneous leishmaniasis (CL).
- To determine if CD26 expression serves as a surrogate marker for interferon-gamma (IFN-gamma) production in CL.
Main Methods:
- Peripheral blood mononuclear cells from CL patients and healthy controls were stimulated with Leishmania antigens.
- Interferon-gamma (IFN-gamma) and interleukin-5 (IL-5) levels were measured.
- CD26 expression on CD4(+) T cells was analyzed using flow cytometry.
Main Results:
- IFN-gamma levels were higher in CL patients than controls, but not significantly different between healing and non-healing groups.
- IL-5 levels were significantly higher in the non-healing CL group compared to others.
- CD26 expression on CD4(+) T cells was significantly increased in both healing and non-healing CL groups compared to controls, with no significant difference between patient groups.
- A weak positive correlation between IFN-gamma and CD26 was observed in the healing group, but not in the non-healing group.
Conclusions:
- Surface CD26 expression is not correlated with the clinical manifestation of CL or IFN-gamma production.
- CD26 is not a valid surrogate marker for IFN-gamma production in cutaneous leishmaniasis.
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