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Published on: May 4, 2018
Redox activity ofHydrodictyon reticulatum plasmalemma vesicles
J Matousková1, L Nespůrková, R Rybová
1Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague.
Folia Microbiologica
|May 8, 2008
Summary
Researchers isolated active plasma membrane vesicles from Hydrodictyon reticulatum. These vesicles demonstrate redox activity, utilizing transmembrane electron flow or external NADH for reduction.
Area of Science:
- Plant cell biology
- Biochemistry
- Membrane transport
Background:
- Hydrodictyon reticulatum possesses mechanically resistant cell walls, posing challenges for isolating cellular components.
- Understanding plasma membrane function requires methods to isolate intact vesicles with preserved biological activity.
Purpose of the Study:
- To prepare plasmalemma vesicles from Hydrodictyon reticulatum while maintaining their redox activity.
- To establish an effective method for isolating plasma-membrane-enriched fractions from algal cells.
Main Methods:
- Combined partial cell-wall enzyme digestion and ultrasonic homogenization for cell disruption.
- Utilized aqueous two-phase polymer system separation for isolating plasma-membrane-enriched microsomal fractions.
- Assessed vesicle integrity and redox activity through electron transfer assays.
Main Results:
- Successfully prepared plasmalemma vesicles from Hydrodictyon reticulatum with preserved redox capabilities.
- The isolation procedure yielded a plasma-membrane-enriched microsomal fraction suitable for functional studies.
- Right-side-out vesicles demonstrated the ability to reduce hexacyanoferrate(III) via transmembrane electron flow or external NADH.
Conclusions:
- The developed methodology enables the isolation of functional plasmalemma vesicles from algae with robust cell walls.
- The findings highlight the redox activity of Hydrodictyon reticulatum plasma membranes, involving electron transport mechanisms.
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