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Published on: May 4, 2018
Redox activity ofHydrodictyon reticulatum plasmalemma vesicles
J Matousková1, L Nespůrková, R Rybová
1Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague.
Abstract:
Plasmalemma vesicles with preserved redox activity were prepared from nets ofHydrodictyon reticulatum. Since the walls are mechanically very resistant, a combination of partial cell-wall enzyme digestion and ultrasonic homogenization had to be used for the disruption of cells. To isolate the plasma-membrane-enriched microsomal fraction separation in an aqueous two-phase polymer system was found to be most suitable. The right-side-out vesicles reduced added hexacyanoferrate(III) by electrons supplied either by a transmembrane flow or from external NADH.
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