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Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in
S H Phadnis1, S Kulakauskas, B R Krishnan
1Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110.
Journal of Bacteriology
|January 1, 1991
Abstract:
An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.