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Related Experiment Video

Updated: Jul 5, 2026

Micro-CT Imaging and Morphometric Analysis of Mouse Neonatal Brains
06:36

Micro-CT Imaging and Morphometric Analysis of Mouse Neonatal Brains

Published on: May 19, 2023

3D micro-CT imaging of the postmortem brain.

Alex de Crespigny1, Hani Bou-Reslan, Merry C Nishimura

  • 1Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Charlestown, MA 02129, USA.

Journal of Neuroscience Methods
|May 9, 2008
PubMed
Summary
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Contrast-enhanced micro-computed tomography (microCT) offers a rapid, cost-effective method for high-resolution 3D brain imaging. This technique provides excellent soft tissue contrast, making it a valuable alternative to microMRI and histopathology for analyzing fixed brain tissue.

Area of Science:

  • Neuroimaging
  • Radiology
  • Biomedical Engineering

Background:

  • Magnetic resonance microscopy (microMRI) is a key tool for non-destructive analysis of fixed brain tissue.
  • X-ray computed tomography (CT) typically shows limited soft tissue contrast in native states.
  • High-resolution 3D imaging of brain tissue requires advanced techniques for detailed analysis.

Purpose of the Study:

  • To explore contrast-enhanced micro-CT (microCT) for high-resolution 3D imaging of fixed normal and pathological brains.
  • To compare the efficacy of microCT with microMRI and standard histopathology.
  • To assess the potential of microCT as an alternative imaging modality for brain tissue analysis.

Main Methods:

  • Brains were immersion stained in iodinated CT contrast media for enhanced contrast.

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  • An optimal iodine concentration of 0.27 M was determined for gray and white matter differentiation.
  • MicroCT imaging was performed on normal and glioma-bearing mouse brains, and results were compared to microMRI and histopathology.

    • Main Results:

    • MicroCT achieved excellent contrast between gray and white matter, identifying numerous anatomical structures.
    • Clear delineation of tumor margins in glioma-bearing brains closely matched histopathology findings.
    • MicroCT-derived tumor volumes showed strong correlation with histopathology volumes, suggesting accuracy in quantification.

    Conclusions:

    • MicroCT image contrast in stained brains is linked to axonal density and myelin content.
    • MicroCT is a relatively rapid and less labor-intensive alternative to histopathology.
    • MicroCT offers a robust, cost-effective alternative to microMRI for non-destructive postmortem brain screening and measurements like tumor volume.